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egfp vamp4  (Addgene inc)


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    Structured Review

    Addgene inc egfp vamp4
    Egfp Vamp4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfp+vamp4/pEGFP+VAMP4+(Plasmid+%2342313)/pm37022307-218-19-31
    Average 93 stars, based on 7 article reviews
    egfp vamp4 - by Bioz Stars, 2026-09
    93/100 stars

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    Plasmid Preparation:

    Article Title: Vesicle-associated membrane protein 2 is a cargo-selective v-SNARE for a subset of GPCRs.
    Article Snippet: VAMP2-pHuji plasmid expressing VAMP2 human isoform1 was a gift from Justin Taraska (National Institutes of Health, Bethesda, MD, USA; #105289; Addgene). .. VAMP4-pHuji plasmid expressing human VAMP4 was cloned by first replacing VAMP2 sequence of VAMP2-pHuji with a VAMP4 sequence from EGFP-VAMP4 (a gift from Thierry Galli, Université Paris Cité, Paris, France; #42313; Addgene) using XhoI and BamHI sites, and then the stop codon and original linker were replaced by VAMP2-pHuji’s linker. .. Lentivirus-compatible FLAG-MOR plasmid was cloned by recombing pENTR-FLAG-MOR with pLenti-CMV-Puro-DEST (a gift from Eric Campeau and Paul Kaufman, UMass Chan Medical School, Worcester, MA, USA; #17452; Addgene) through LR reaction following a published protocol (Campeau et al., 2009).

    Expressing:

    Article Title: Vesicle-associated membrane protein 2 is a cargo-selective v-SNARE for a subset of GPCRs.
    Article Snippet: VAMP2-pHuji plasmid expressing VAMP2 human isoform1 was a gift from Justin Taraska (National Institutes of Health, Bethesda, MD, USA; #105289; Addgene). .. VAMP4-pHuji plasmid expressing human VAMP4 was cloned by first replacing VAMP2 sequence of VAMP2-pHuji with a VAMP4 sequence from EGFP-VAMP4 (a gift from Thierry Galli, Université Paris Cité, Paris, France; #42313; Addgene) using XhoI and BamHI sites, and then the stop codon and original linker were replaced by VAMP2-pHuji’s linker. .. Lentivirus-compatible FLAG-MOR plasmid was cloned by recombing pENTR-FLAG-MOR with pLenti-CMV-Puro-DEST (a gift from Eric Campeau and Paul Kaufman, UMass Chan Medical School, Worcester, MA, USA; #17452; Addgene) through LR reaction following a published protocol (Campeau et al., 2009).

    Clone Assay:

    Article Title: Vesicle-associated membrane protein 2 is a cargo-selective v-SNARE for a subset of GPCRs.
    Article Snippet: VAMP2-pHuji plasmid expressing VAMP2 human isoform1 was a gift from Justin Taraska (National Institutes of Health, Bethesda, MD, USA; #105289; Addgene). .. VAMP4-pHuji plasmid expressing human VAMP4 was cloned by first replacing VAMP2 sequence of VAMP2-pHuji with a VAMP4 sequence from EGFP-VAMP4 (a gift from Thierry Galli, Université Paris Cité, Paris, France; #42313; Addgene) using XhoI and BamHI sites, and then the stop codon and original linker were replaced by VAMP2-pHuji’s linker. .. Lentivirus-compatible FLAG-MOR plasmid was cloned by recombing pENTR-FLAG-MOR with pLenti-CMV-Puro-DEST (a gift from Eric Campeau and Paul Kaufman, UMass Chan Medical School, Worcester, MA, USA; #17452; Addgene) through LR reaction following a published protocol (Campeau et al., 2009).

    Sequencing:

    Article Title: Vesicle-associated membrane protein 2 is a cargo-selective v-SNARE for a subset of GPCRs.
    Article Snippet: VAMP2-pHuji plasmid expressing VAMP2 human isoform1 was a gift from Justin Taraska (National Institutes of Health, Bethesda, MD, USA; #105289; Addgene). .. VAMP4-pHuji plasmid expressing human VAMP4 was cloned by first replacing VAMP2 sequence of VAMP2-pHuji with a VAMP4 sequence from EGFP-VAMP4 (a gift from Thierry Galli, Université Paris Cité, Paris, France; #42313; Addgene) using XhoI and BamHI sites, and then the stop codon and original linker were replaced by VAMP2-pHuji’s linker. .. Lentivirus-compatible FLAG-MOR plasmid was cloned by recombing pENTR-FLAG-MOR with pLenti-CMV-Puro-DEST (a gift from Eric Campeau and Paul Kaufman, UMass Chan Medical School, Worcester, MA, USA; #17452; Addgene) through LR reaction following a published protocol (Campeau et al., 2009).



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    egfp vamp4  (Addgene inc)
    93
    Addgene inc egfp vamp4
    Egfp Vamp4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfp+vamp4/pEGFP+VAMP4+(Plasmid+%2342313)/pm37022307-218-19-31
    Average 93 stars, based on 1 article reviews
    egfp vamp4 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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